Total Flavonoids of Astragalus Membranaceus Protect Against 1-Methyl-4-Phenyl-1,2,3,6-Tetrahydropyridine-Induced Neurotoxicity in Mice by Inhibiting Ferroptosis through SLC7A11/GPX4 Signaling Pathway

Parkinson's disease (PD) is a common neurodegenerative disorder with no cure. Astragalus membranaceus used in Chinese culture as food supplement to boost immunity. The present study aimed explore the neuroprotective effects of total flavonoids extracted from A. (TFA) and their protective mechanisms. TFA offered neuroprotection against 1-methyl-4-phenyl-1,2,3,6 -tetrahydropyridine (MPTP) mouse model Parkinsonism, by improving behavior performance gait analysis pole test, inhibiting decline tyrosine hydroxylase (TH) positive neurons TH protein expression Substantia Nigra mice. also prevented 1-methyl-4- phenylpyridinium (MPP+)-induced neurotoxicity SH-SY5Y cells, increasing GSH GSH/GSSG ratio, reducing reactive oxygen species. In addition, were associated its ability restore MPTP/MPP+-induced downregulation SLC7A11 glutathione peroxidase 4 (GPX-4). conclusion, we demonstrated that exerted significant MPTP/MPP+ induced neurodegeneration ferroptosis through regulation SLC7A11/GPX-4 axis, suggesting use possible prevention PD.